FM Minimal Medium Size:1kg Tryptone 8 Yeast extract 5

Tryptone 8 Yeast extract 5 NaCl 5 Agar 15 Final pH 7

Luria Agar

These Amino acids form a basis for many “Drop-Out” mixtures to select for auxotrophic requirements and transformants

The FM drop-out mixture

glycerol is not fermented to acetic acid

FM Minimal Medium Size:1kg Tryptone 8 Yeast extract 5This media is based on well defined formulations of mineral salts, vitamins and amino acids. Two commonly used synthetic defined minimal Dictystelium Discoideum media are FM and SIH. FM medium developed by Franke and Kessin is used for transformation of Dictyostelium Discoideum and genomic studies. This formulation supports the growth of most strains that are capable of growing on HL5. SIH medium is a newly developed modification of FM with regards to