SIH Medium Size:1kg 2 at 25°C Suspend 28

2 at 25°C Suspend 28 gram powdered medium in 1 litre distilled water

98 Agar 7 Final pH 7

2 K2HPO4 9

DNA replication inhibitor causing deoxyribonucleotide depletion

This formulation supports the growth of most strains that are capable of growing on HL5

SIH Medium Size:1kg 2 at 25°C Suspend 28SIH medium, developed by Hanh, Friehs and Flaschel (2004), is the next step in the development of synthetic media designed to grow D. discoideum in high cell densities. SIH medium is an improved version of FM medium, as developed by Frank and Kessin (1977). The main difference between SIH and FM is the novel addition of Aspartic acid (1. 1 mM) and increased levels of Lysine (8. 5 mM) and Tryptophane (1. 7 mM). Concentrations of Cysteine, Glutamic