TAE Buffer 50X Size:5L Component g/l Peptone 14 Yeast

Component g/l Peptone 14 Yeast Extract 7 Glucose 13

The pH (and pKa at ionic strength I≠0) of the buffer solution changes with concentration and temperature

0002% Sulfate (SO4) <0

2 Glucose 0

2 at 25°C Suspend 25 gram powdered medium in 1 litre distilled water

TAE Buffer 50X Size:5L Component g/l Peptone 14 Yeast50 X sterile stock solution Dilute 20ml TAE Buffer 50X with 980ml deionised water to make 1 litre of TAE Buffer. Final concentration is 40mM Tris Base, 20mM Glacial Acetic Acid and 1mM Na2EDTA. Specifications TRIS 2. 0 M 242. 0 g L Acetic Acid 1. 0 M 60. 0 g L Na2EDTA 0. 05 M 30. 3 g L Store at room temperature Keep away from light